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a conditional origin of replication which happens to be inactive from the focused receiver bacterial mobile but is Energetic in a donor bacterial mobile, and
in a very most most popular embodiment, the vector according to the invention, 點此進一步了解 ideally A part of a delivery car, does not comprise any restriction site.
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By “in trans” is supposed herein that said protein, peptid, RNA, nucleic acid, molecule or any mix thereof isn't encoded on the identical nucleic acid molecule since the a single comprising the origin of replication. In a specific embodiment, stated protein, peptid, RNA, nucleic acid, molecule or any mixture thereof is encoded with a chromosome or on a plasmid.
In a certain embodiment, the qualified receiver bacteria are linked to the triggering, progression or aggravation of CNS linked condition in the host. In a certain embodiment, the targeted receiver germs are associated with the resistance from the host in direction of therapies versus an infection, tumor, neurodegenerative condition, CNS linked disease, autoimmune illness, and/or cancer.
This is completely distinctive to get a bacterial ORI, because it would suggest that It might be Lively Obviously and constitutively.
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“Cas9” can So denote a modified protein, for example truncated to eliminate domains on the protein that aren't important for the predefined functions of the protein, specifically the domains that aren't needed for interaction Using the gRNA(s).
normally, stated presented molecule the manufacture of that is being stopped incorporates a damaging effect on stated host organism.
The choice of promoter around the vector according to the present invention can Hence be manufactured based upon the micro organism to target.
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260、细菌噬菌体可选自肌尾噬菌体科(非限制性地比如以下属：cp220病毒、cp8病毒、ea214病毒、felixo1病毒、moogle病毒、susp病毒、hp1病毒、p2病毒、kay病毒、p100病毒、silvia病毒、spo1病毒、tsarbomba病毒、twort病毒、cc31病毒、jd18病毒、js98病毒、kp15病毒、moon病毒、rb49病毒、rb69病毒、s16病毒、schizot4病毒、sp18病毒、t4病毒、cr3病毒、se1病毒、v5病毒、abouo病毒、agate病毒、agrican357病毒、ap22病毒、arv1病毒、b4病毒、bastille病毒、bc431病毒、bcep78病毒、bcepmu病毒、biquarta病毒、bxz1病毒、cd119病毒、cp51病毒、cvm10病毒、eah2病毒、el病毒、hapuna病毒、jimmer病毒、kpp10病毒、m12病毒、machina病毒、martha病毒、msw3病毒、mu病毒、myohalo病毒、nit1病毒、p1病毒、pakpuna病毒、pbuna病毒、phikz病毒、rheph4病毒、rsl2病毒、rsluna病毒、secunda5病毒、sep1病毒、spn3病毒、svuna病毒、tg1病毒、vhml病毒和wph病毒)。
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